The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).
How does insulin stimulate the uptake of glucose?
Insulin increases glucose uptake mainly by enriching the concentration of Glut4 proteins at the plasma membrane, rather than by increasing the intrinsic activity of the transporter (2,3).
What type of transport is glucose uptake?
facilitated diffusion
The two ways in which glucose uptake can take place are facilitated diffusion (a passive process) and secondary active transport (an active process which on the ion-gradient which is established through the hydrolysis of ATP, known as primary active transport).
How is glucose uptake measured?
The Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P.
Where does glucose uptake occur?
Insulin’s actions on glucose uptake are predominantly mediated by GLUT 4, which is found in muscle, fat, and heart, where glucose uptake is insulin-mediated. GLUT 4 is usually found in intracellular vesicles, but in adults, insulin stimulates the migration of GLUT 4 transporters to the plasma membrane.
What is radiolabeled glucose?
Chemical • Deoxy-D-glucose (DOG) is a glucose analog that is readily transported into most cells, is phosphorylated and trapped by the cells (unidirectional transport), and cannot be further metabolized. It is the most-common chemical used in glucose uptake assays.
What is insulin glucose uptake?
1. Insulin-Dependent Glucose Uptake Mediated by Subcellular Redistribution of the Glucose Transporter. Insulin is an anabolic hormone that acts on various target tissues, including the liver, skeletal muscle, and fat tissue, regulating the blood glucose level [1,2,3,4].
Is insulin required for glucose uptake?
Insulin Is not Required for Glucose Uptake Into Cells.
Which glucose transporter is insulin dependent?
GLUT-IV is insulin dependent and is responsible for majority of glucose transport into muscle and adipose cells in anabolic conditions.
Is insulin active or passive transport?
Insulin triggers GLUT4 to insert into the membranes of these cells so that glucose can be taken in from the blood. Since this is a passive mechanism, the amount of sugar entering our cells is proportional to how much sugar we consume, up to the point that all our channels are being used (saturation).
How does glucose uptake assay work?
The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). Simple, homogeneous protocol: After addition of 2DG, there are no wash steps—all steps are additions.
Why is glucose uptake assay done?
Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, and cancer.
