Sam68 is a known sequence-specific RNA binding protein that regulates alternative splicing events during the cell cycle and apoptosis. Sam68 has also been shown to influence transcription, but the molecular mechanism remains undefined.
What is the purpose of alternative RNA splicing?
The overall function of alternative splicing is to increase the diversity of the mRNA expressed from the genome. Due to the combinatorial control mechanisms that regulate alternative exon recognition, splicing programs coordinate the generation of mRNA isoforms from multiple genes.
What is an example of alternative splicing?
Alternative splicing is a powerful means of controlling gene expression and increasing protein diversity. The best example is the Drosophila Down syndrome cell adhesion molecule (Dscam) gene, which can generate 38,016 isoforms by the alternative splicing of 95 variable exons.
What is alternative splicing and what does it lead to?
Alternative splicing is the process of selecting different combinations of splice sites within a messenger RNA precursor (pre-mRNA) to produce variably spliced mRNAs. These multiple mRNAs can encode proteins that vary in their sequence and activity, and yet arise from a single gene.
What does alternative splicing result in?
Alternative splicing is a molecular mechanism that modifies pre-mRNA constructs prior to translation. This process can produce a diversity of mRNAs from a single gene by arranging coding sequences (exons) from recently spliced RNA transcripts into different combinations.
How does alternative RNA splicing regulate transcription?
Notably, alternative splicing allows the human genome to direct the synthesis of many more proteins than would be expected from its 20,000 protein-coding genes. The production of alternatively spliced mRNAs is regulated by a system of trans-acting proteins that bind to cis-acting sites on the primary transcript itself.
What is the difference between RNA splicing and alternative RNA splicing?
The main difference between RNA splicing and alternative splicing is that the RNA splicing is the process of splicing the exons of the primary transcript of mRNA whereas the alternative splicing is the process of producing differential combinations of exons of the same gene.
What is alternative RNA splicing BBC Bitesize?
Alternative RNA splicing The benefit of RNA splicing is that one gene can produce many different proteins as a result of what segments are treated as introns and exons.
What does alternative mRNA Splicing generate?
Alternative splicing generates transcriptome diversity and enables gene regulation. It can generate mRNAs that encode proteins with different or even opposite functions.
What is alternative splicing of pre-mRNA?
Alternative pre-mRNA splicing is the process by which multiple mRNAs can be generated from the same pre-mRNA by the differential joining of 5′ and 3′ splice sites. For example, exons can be extended or shortened, skipped or included, and introns can be removed or retained in the mRNA22.
What regulates alternative splicing?
Splicing is regulated by trans-acting proteins (repressors and activators) and corresponding cis-acting regulatory sites (silencers and enhancers) on the pre-mRNA. However, as part of the complexity of alternative splicing, it is noted that the effects of a splicing factor are frequently position-dependent.
What’s the difference between splicing and alternative splicing?
