Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.
What is constitutive promoter?
Constitutive promoters are defined as promoters active in vivo in all circumstances, and, on the other hand, inducible promoters are switched ON and OFF by transcription factors depending on the in vivo conditions (Shimada et al., 2014).
What is Wpre sequence?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of genes delivered by viral vectors.
What is Syn promoter?
Synapsin (SYN) promoter in the vector drives expression of green fluorescent protein (GFP) only in neurons as verified by colocalization with NeuN but not GFAP. Primary murine neocortical cultures containing both neurons and astrocytes were transduced with the lentiviral vector SYN-GFP at an MOI of 5.
What is a U6 promoter?
U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA (shRNA) expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector.
What is an endogenous promoter?
Endogenous promoters for constitutive and efficient expression of transgenes are generally obtained from non-coding regions upstream of a transcription initiation site of genes [46], that are associated with high and steady expression throughout most experimental conditions.
What does the WPRE do?
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed downstream of the transgene, proximal to the polyadenylation signal.
What is SYN1 gene?
SYN1 (Synapsin I) is a Protein Coding gene. Diseases associated with SYN1 include Epilepsy, X-Linked, With Variable Learning Disabilities And Behavior Disorders and Intellectual Developmental Disorder, X-Linked 50. Among its related pathways are Neurotransmitter Release Cycle and Synaptic Vesicle Pathway.
What is the function of the EF-1α promoter?
The EF-1α promoter maintains high-level transgene expression from episomal vectors in transfected CHO-K1 cells
What is the difference between CMV and eEF1A1 promoter?
In comparison with the CMV promoter, the eukaryotic translation elongation factor 1 α (EF-1α, gene symbol EEF1A1) promoter increased the transfection efficiency, the transgene expression, the proportion of expression-positive clones and the copy number of the episomal vector in long-term culture.
Is there an ef1α promoter for submandibular glands?
Although, all are called the EF1α promoter, the functional activity from one commercially-obtained promoter was markedly lower in four different cell lines in vitro and in vivo in rat submandibular glands.
What is eEF1A1 phosphorylation by TGF-beta?
Phosphorylation of eEF1A1 by TbetaR-I is a novel regulatory mechanism that provides a direct link to regulation of protein synthesis by TGF-beta, as an important component in the TGF-beta-dependent regulation of protein synthesis and cell proliferation
